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KMID : 0861120140180010125
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Korean Journal of Oriental Preventive Medicine
2014 Volume.18 No. 1 p.125 ~ p.138
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Synergic Effects of Mixed Formula Consisted of Polycan and Calcium-gluconate on the Experimental Periodontitis and Alveolar Bone Loss in Rats
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Lee Won-Ho
Kim Kyung-Hu
Ku Sae-Kwang
Kang Su-Jin
Lee Young-Joon
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Abstract
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Objective : Polycan, exopolymers purified from Aureobasidium pullulans SM-2001 and calcium gluconate havebeen showed favorable inhibitory effects on the periodontitis and related alveolar bone losses through antioxidantand anti-inflammatory activities, respectively. In the present study, we intended to observe the possiblesynergic effects of mixed formula consisted of Polycan and calcium gluconate on ligation-induced experimentalperiodontitis and related alveolar bone losses in rats, and to select the fittest compositions for furtherdeveloping as effective agents to ameliorate periodontal diseases.
Method : Experiments were conducted as two separated two tests ? first is synergic effects of Polycan andcalcium gluconate 1:1, 1:9 and 9:1 mixtures, and second is 1:99, 2:98, 4:96, 8:92 and 1:9 mixtures. Experimentalperiodontal diseases were induced by ligature placed around the cervix of upper left incisior teethof rats. One day after ligation placements, 200mg/kg of each single or mixed formulas of Polycan or/andcalcium gluconate were orally administered for 10 days. The changes on the alveolar bone loss index andmaxillary bone mineral density (BMD) were observed for detecting alveolar bone losses, and for anti-inflammatoryeffects, myeloperoxidase (MPO) activities and proinflammatory cytokine (tumor necrosis factor; TNF-α) contents were also evaluated in gingival tissues around ligature placed incisior teeth. The results of mixtureswere compared with those of singe Polycan and calcium gluconate treated rat.
Results : Each single or mixed formulas of Polycan or/and calcium gluconate favorably and significantlyinhibited the inflammatory changes. The inhibitory effects of mixed formula consisted of Polycan and calciumgluconate 1:9 showed against periodontitis and related alveolar bone losses as compared with those ofeach Polycan and calcium gluconate single formula (p<0.05). In second experiment, Polycan and calciumgluconate 2:98, 4:96, 8:92 and 1:9 mixed formulas also showed significant increased anti-inflammatoryand inhibitory effects against alveolar bone losses as compared with those of each single formula. Amongthem, Polycan and calcium gluconate 2:98 showed the highest efficacy against to ligation-induced experimentalperiodontitis and related alveolar bone losses.
Conclusion : The results obtained in this study suggest that appropriated mixtures of Polycan and calciumgluconate showed synergic inhibitory effects against ligation-induced experimental periodontitis and relatedalveolar bone losses in rats. Moreover, Polycan and calcium gluconate 2:98 showed the highest efficaciesin this experiment, suggesting the fittest composition for further developing as effective agents to ameliorateperiodontal diseases.
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KEYWORD
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Polycan, Calcium gluconate, Synergic effects, Periodontitis, Alveolar bone loss, Rat
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